Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 3. VAC alleviated the renal inflammatory response and oxidative stress in T2DM mice (A) MDA contents in diabetic kidney tissues. n=6. (B) GSH-Px activity in diabetic kidney tissues. n=6. (C) Averaged fluorescence intensity of DHE fluorescence in diabetic kidney tissues. n=6. (D) Averaged fluorescence intensity of DCFH-DA fluorescence staining of diabetic kidney tissues. n=6. (E) DHE fluorescence staining or DCFH-DA fluorescence staining of diabetic kidney tissues. Scale bar: 100 μm. (F) F4/80 staining of diabetic kidney tissues. Scale bar: 50 μm, and the average fluorescence intensity of F4/80-expressing diabetic kidney tissues is shown. (G‒M) Representative blot images and quantitative analysis of phosphorylated NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. n=4. *P<0.05, **P<0.01, ***P<0.001 vs Ctrl. #P<0.05, ##P<0.01, ###P<0.001 vs DN.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Activity Assay, Fluorescence, Staining, Expressing

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 5. VAC alleviated the inflammatory response and oxidative stress in diabetic kidneys HK-2 cells were preincubated with 5 μM VAC for 12 h, followed by exposure to 35 mM HG for 48 h. (A–C) Relative mRNA levels of IL-1β, VCAM-1 and COX2. (D-F) DHE fluorescence staining or DCFH-DA fluorescence staining of HK-2 cells. Scale bar: 100 μm. (G–K) Relative mRNA levels of Nrf2, catalase, SOD3, SOD2 and SOD1. (L–R) Representative blot images and quantitative analysis of phosphorylated NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. *P<0.05, **P<0.01, ***P<0.001 vs NG. #P<0.05, ##P<0.01, ###P<0.001 vs HG. n=4.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Fluorescence, Staining

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 8. VAC ameliorated fibrosis, the inflammatory response and oxidative stress in HG-induced HK-2 cells exposed to the EGFR inhibitor AG1478 or the ERK inhibitor U0126 (A–D) Relative mRNA levels of collagen-1, TGF-β1, α-SMA and E-cadherin in HK-2 cells. (E–G) Relative mRNA levels of IL-1β, VCAM-1 and COX-2 in HK-2 cells. (H) Averaged fluorescence intensity of DHE fluorescence in HK-2 cells. (I) DHE staining was performed on HG-induced HK-2 cells. Scale bar: 100 μm. (J–P) Representative blot images and quantitative analysis of phosphorylated and total NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. *P<0.05, **P<0.01, ***P<0.001 vs NG. #P<0.05, ##P<0.01, ###P<0.001 vs HG. n=4.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Fluorescence, Staining

Journal: PLoS ONE

Article Title: Illuminating the effect of beneficial blue light and ROS-modulating enzymes in Dupuytren’s disease

doi: 10.1371/journal.pone.0253777

Figure Lengend Snippet: Antibodies.

Article Snippet: SOD1 , StressMarq Biosciences , SPC-116-D , Rabbit , 1:1000 in 5% BSA.

Techniques:

Journal: PLoS ONE

Article Title: Illuminating the effect of beneficial blue light and ROS-modulating enzymes in Dupuytren’s disease

doi: 10.1371/journal.pone.0253777

Figure Lengend Snippet: GPX activity DD fibroblasts (A and B). There is no significant difference of GPX activity on day 3 (A) and 5 (B). Bars represent mean ± SD of individual experiments indicated (n = 10). Relative protein expression of SOD1 in DD fibroblasts (C and D). Blue light irradiation caused no significant effect on DD fibroblasts on day 3 and 5. * p ≤ 0.05. Bars represent mean ± SD of individual experiments indicated (n = 8). Relative protein expression of NOX4 in DD fibroblasts (E and F). TGF-β1 caused an elevated NOX4 expression in tendency, and the blue light irradiation further increased this effect on day 3, even though this effect was not significant. On day 5 the effect was reduced. Bars represent mean ± SD of individual experiments indicated (n = 8).

Article Snippet: SOD1 , StressMarq Biosciences , SPC-116-D , Rabbit , 1:1000 in 5% BSA.

Techniques: Activity Assay, Expressing, Irradiation

Journal: International Journal of Molecular Sciences

Article Title: Fe 3 O 4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D -Galactose-Induced Aged Mice

doi: 10.3390/ijms23126463

Figure Lengend Snippet: ( A ) Measurement of the MDA content. ( B ) Measurement of SOD activity. ( C ) Western blotting analysis of SOD1, SOD2, and catalase in the hippocampus of the control group ( D -gal-, Mel-, pFe 3 O 4 -); 100 mg/kg D -gal-treated group ( D -gal+, Mel-, pFe 3 O 4 -); 50 mg/kg Melatonin+100 mg/kg D -gal-treated group ( D -gal+, Mel+, pFe 3 O 4 -); 10 μg/mL PEG-Fe 3 O 4 nanozyme-treated group ( D -gal-, Mel-, pFe 3 O 4 +); 10 μg/mL PEG-Fe 3 O 4 nanozymes+100 mg/kg D -gal-treated group ( D -gal+, Mel-, pFe 3 O 4 +). ( D – F ) ROD presented as percentages of the immunoblot band ( n = 7 per group; * p < 0.05, ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the D -gal group). Bars indicate the means ± SDs.

Article Snippet: Then, the membranes were incubated with rabbit antibodies to zonula occludens-1 (ZO-1) (1:1000; Thermo Fisher Scientific), Claudin5 (1:1000; Abcam, Cambridge, MA, USA), SOD1 and SOD2 (1:1000; Arigo, Burlington, ON, Canada), catalase (1:1000; Abcam), Bcl-2 (1:1000; Santa Cruz), caspase-3 (1:1000; Cell Signaling Technology, Danvers, MA, USA), cleaved caspase-3 (1:1000; Cell Signaling Technology), Beclin-1 (1:1000; Santa Cruz), microtubule-associated Protein 1A/1B Light chain 3 (LC3II/I) (1:1000; Cell Signaling Technology), Autophagy Related 7 (Atg7) (1:1000; Cell Signaling Technology), protein kinase B (Akt) (1:1000; Cell Signaling Technology), phospho (p)-Akt (1:1000; Cell Signaling Technology), mammalian target of rapamycin (mTOR) (1:1000; Cell Signaling Technology), p-mTOR (1:1000; Santa Cruz), and β-actin (1:1000; Arigo) overnight at 4 °C with gentle agitation and washed three times for 5 min each time with 15 mL TBST, and then subsequently exposed to secondary goat anti-rabbit IgG (Santa Cruz) at room temperature for 2 h, and Super Signal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used for protein detection.

Techniques: Activity Assay, Western Blot